1SCH
PEANUT PEROXIDASE
Experimental procedure
Source type | ROTATING ANODE |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Collection date | 1991-09 |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.100, 97.200, 146.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.560 |
R-factor | 0.199 |
Rwork | 0.199 |
R-free | 0.26200 |
RMSD bond length | 0.006 |
RMSD bond angle | 20.619 * |
Data reduction software | XENGEN ((HOWARD) |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 2.560 | 2.560 * |
Rmerge | 0.086 | |
Total number of observations | 56127 * | |
Number of reflections | 19674 | |
Completeness [%] | 86.0 * | 41 * |
Redundancy | 2.85 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.7 | pH 6.7 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 12 (%) | |
2 | 1 | drop | enzyme solution | 15 (mg/ml) | |
3 | 1 | drop | sodium phosphate | 0.35 (M) | |
4 | 1 | drop | PEG4000 | 12 (%) | |
5 | 1 | drop | n-octyl-beta-D-glucoside | 35 (%) |