1SBM
Crystal Structure of Reduced Mesopone cytochrome c peroxidase (R-isomer)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 116 |
Detector technology | IMAGE PLATE |
Collection date | 2003-06-10 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54178 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.826, 75.666, 50.974 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 1.720 - 1.690 |
Rwork | 0.181 |
R-free | 0.20040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s73 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.272 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.720 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.062 | 0.597 |
Number of reflections | 47110 | |
<I/σ(I)> | 20.98 | 1.8 |
Completeness [%] | 99.0 | 96.9 |
Redundancy | 16.32 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 2-methyl-2,4-pentanediol (MPD), 100mM potassium phosphate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |