1SBH
SUBTILISIN BPN' 8397+1 (E.C. 3.4.21.14) MUTANT (M50F, N76D, G169A, Q206C, N218S, K256Y)
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1994-07-10 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.560, 79.530, 37.250 |
| Unit cell angles | 90.00, 114.48, 90.00 |
Refinement procedure
| Resolution | 17.000 - 1.800 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.19200 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.694 |
| Data reduction software | TEXSAN |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 17.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.113 |
| Number of reflections | 21228 |
| Completeness [%] | 100.0 |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.5-5 (mg/ml) | |
| 2 | 1 | drop | sodium cacodylate | 5 (mM) | |
| 3 | 1 | drop | PEG3400 | 8.5 (%(w/v)) | |
| 4 | 1 | drop | 2-propanol | 8.5 (%(v/v)) | |
| 5 | 1 | drop | sodium citrate | 50 (mM) | |
| 6 | 1 | reservoir | PEG3400 | 17 (%(w/v)) | |
| 7 | 1 | reservoir | 2-propanol | 17 (%(v/v)) | |
| 8 | 1 | reservoir | sodium citrate | 100 (mM) |
