1SBD
SOYBEAN AGGLUTININ COMPLEXED WITH 2,4-PENTASACCHARIDE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 287 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-10-09 |
| Detector | SIEMENS |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 144.600, 144.600, 107.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.520 |
| Rwork | 0.205 |
| Structure solution method | RIGID BODY REFINEMENT VS 1SBA |
| RMSD bond length | 0.017 |
| RMSD bond angle | 18.573 * |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | X-PLOR |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.670 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.140 | 0.411 |
| Total number of observations | 112590 * | |
| Number of reflections | 18788 | |
| <I/σ(I)> | 7.5 | 2.7 |
| Completeness [%] | 82.0 | 49 * |
| Redundancy | 5.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 | 4 * | Dessen, A., (1995) Biochemistry, 34, 4933. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | SBA | 17 (mg/ml) | |
| 10 | 1 | reservoir | 0.5 (M) | ||
| 2 | 1 | drop | sugar | 0.755 (mM) | |
| 3 | 1 | drop | HEPES | 0.1 (M) | |
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | drop | 1 (mM) | ||
| 6 | 1 | drop | 0.375 (M) | ||
| 7 | 1 | reservoir | HEPES | 0.1 (M) | |
| 8 | 1 | reservoir | 1 (mM) | ||
| 9 | 1 | reservoir | 1 (mM) |
