1SAW

X-ray structure of homo sapiens protein FLJ36880

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-09-19
Detector	MARRESEARCH
Wavelength(s)	0.9083
Spacegroup name	P 21 21 21
Unit cell lengths	50.792, 77.239, 114.195
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.650 - 2.200
Rwork	0.204
R-free	0.23876
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SWISS MODEL (PDB ENTRIES 1NR9 and 1GTT)
RMSD bond length	0.008
RMSD bond angle	1.157
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4 ((MOLREP))
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.071	0.329
Number of reflections	21327
<I/σ(I)>	21.2	3.4
Completeness [%]	91.0	60.8
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	PEG4000, Magnesium chloride, Na HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K