1SA5

Rat protein farnesyltransferase complexed with FPP and BMS-214662

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-04
Detector	ADSC QUANTUM 315
Spacegroup name	P 61
Unit cell lengths	170.469, 170.469, 69.028
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.210 - 2.600
R-factor	0.188
Rwork	0.188
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d8d
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	35102
<I/σ(I)>	11.8	3.7
Completeness [%]	98.7	97.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.7	290	14% PEG 8000, 400 mM ammonium acetate pH 5.7, 20 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 290K, pH 5.70