1SA4

human protein farnesyltransferase complexed with FPP and R115777

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-10-24
Detector	ADSC QUANTUM 4
Spacegroup name	P 61
Unit cell lengths	178.442, 178.442, 64.458
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.880 - 2.100
R-factor	0.166
Rwork	0.166
R-free	0.19500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jcq
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	66907
<I/σ(I)>	10.6	3
Completeness [%]	96.6	92

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.7	290	14% PEG 8000, 400 mM ammonium acetate pH 5.7, 20 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 290K, pH 5.70