1S9H

Crystal Structure of Adeno-associated virus Type 2 Rep40

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	109
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9812, 0.9810, 0.9583
Spacegroup name	P 65
Unit cell lengths	126.294, 126.294, 97.894
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.970 - 2.400
R-factor	0.227
Rwork	0.227
R-free	0.29300
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400	2.400
Rmerge		0.373
Number of reflections	35212
<I/σ(I)>	16.8	1.42
Completeness [%]		84

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	PEG 8000, Sodium acetate, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	PEG 8000, Sodium acetate, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K