1S98

E.coli IscA crystal structure to 2.3 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	93
Detector technology	CCD
Collection date	2002-12-20
Detector	ADSC QUANTUM 4
Spacegroup name	P 32 2 1
Unit cell lengths	66.252, 66.252, 150.850
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.920 - 2.300
R-factor	0.215
Rwork	0.212
R-free	0.24967
Structure solution method	SAD
RMSD bond length	0.038
RMSD bond angle	2.507
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.350
High resolution limit [Å]	2.300	2.300
Number of reflections	20165
<I/σ(I)>	7.2	2
Completeness [%]	99.9	99.6
Redundancy	8.2	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	296	NaPhosphate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 23K