1S7Y
Crystal structure of the GluR6 ligand binding core in complex with glutamate at 1.75 A resolution orthorhombic form
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.96746, 0.97892, 0.97930 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.101, 91.187, 105.447 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.510 - 1.750 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.24300 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.047 | 0.177 |
| Number of reflections | 56491 | |
| <I/σ(I)> | 20.6 | 6.12 |
| Completeness [%] | 99.1 | 98.5 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 14% PEG 3350, 10 Citrate, 20 NaCl, 1 EDTA, 10 NaGlutamate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
