1S7X
Crystal structures of the murine class I major histocompatibility complex H-2Db in complex with LCMV-derived gp33 index peptide and three of its escape variants
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-03-12 |
Detector | MARRESEARCH |
Wavelength(s) | 1.007 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 91.897, 123.318, 100.201 |
Unit cell angles | 90.00, 102.82, 90.00 |
Refinement procedure
Resolution | 19.800 - 2.410 |
Rwork | 0.203 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n5a |
RMSD bond length | 0.012 |
RMSD bond angle | 1.250 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.800 | 2.540 |
High resolution limit [Å] | 2.410 | 2.410 |
Number of reflections | 83027 | |
Completeness [%] | 98.2 | 89.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Ammonium sulfate, Tris , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |