1S7U
Crystal structures of the murine class I major histocompatibility complex H-2Db in complex with LCMV-derived gp33 index peptide and three of its escape variants
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.389, 123.343, 99.392 |
| Unit cell angles | 90.00, 103.17, 90.00 |
Refinement procedure
| Resolution | 72.700 - 2.200 |
| R-factor | 0.21569 |
| Rwork | 0.213 |
| R-free | 0.26222 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n5a |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.203 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.700 | 2.420 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 108769 | |
| Completeness [%] | 99.0 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Ammonium sulfate, Tris , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
