1S7L

RimL- Ribosomal L7/L12 alpha-N-protein acetyltransferase in complex with Coenzyme A (CoA-Cys134 Disulfide)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	77
Detector technology	IMAGE PLATE
Collection date	2003-03-03
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	92.920, 92.920, 55.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.193
Rwork	0.193
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1s7f
RMSD bond length	0.022
RMSD bond angle	2.000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	10924
<I/σ(I)>	20.8
Completeness [%]	96.6	93.7
Redundancy	5	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	vapor diffusion under oil	8	293	pentaerythritiol ethoxylate 797, triethanolamine, ammonium sulfate, acetylcoenzyme A, DTT, pH 8, vapor diffusion under oil, temperature 293K