1S7J

Crystal structure of phenazine biosynthesis protein PhzF family (Enterococcus faecalis)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-12-08
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	60.800, 85.740, 105.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.920 - 2.300
R-factor	0.229
Rwork	0.229
R-free	0.27300
Structure solution method	MIR
RMSD bond length	0.011
RMSD bond angle	1.500
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.073	0.176
Number of reflections	25009
<I/σ(I)>	16.2	3.2
Completeness [%]	99.1	93.9
Redundancy	18	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	290	100 mM HEPES-Na, pH7.5, 27% PEG 3350, 200 mM MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 290K, pH 7.50