1S7H

Structural Genomics, 2.2A crystal structure of protein YKOF from Bacillus subtilis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-03-10
Detector	SBC-2
Wavelength(s)	0.9795, 0.9798
Spacegroup name	P 21 21 2
Unit cell lengths	169.740, 55.078, 85.493
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.200 - 2.200
R-factor	0.221
Rwork	0.221
R-free	0.28100
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.300
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.089	0.400
Number of reflections	81572
<I/σ(I)>	23.4	3.85
Completeness [%]	99.0	95.2
Redundancy	9.2	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.2M MgCl2 Hexahydrate, 0.1M Tris , 25% PEG 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K