1S7F
RimL- Ribosomal L7/L12 alpha-N-protein acetyltransferase crystal form I (apo)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 77 |
Detector technology | IMAGE PLATE |
Collection date | 2002-12-13 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 68.400, 68.400, 89.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.24200 |
Structure solution method | SIRAS |
RMSD bond length | 0.022 |
RMSD bond angle | 2.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 14015 | |
<I/σ(I)> | 18.2 | |
Completeness [%] | 92.7 | 83.5 |
Redundancy | 3.2 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | vapor diffusion under oil | 7.8 | 293 | triethanolamine, ammonium sulfate, sodium malonate, pH 7.8, vapor diffusion under oil, temperature 293K |