1S70
Complex between protein ser/thr phosphatase-1 (delta) and the myosin phosphatase targeting subunit 1 (MYPT1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2003-08-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 75.188, 75.188, 241.753 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.670 - 2.700 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.29300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1FJM for molecule 1 and 3 ankyrin repeats from 1IHB for molecule 2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.700 | 2.910 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.093 | 0.430 |
| Number of reflections | 18135 | |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 91.1 | 65.1 |
| Redundancy | 13.6 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 5000 monomethylether, glycerol, NH4Cl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
