1S60
Aminoglycoside N-Acetyltransferase AAC(6')-Iy in Complex with CoA and N-terminal His(6)-tag (crystal form 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 77 |
Detector technology | IMAGE PLATE |
Collection date | 2002-06-26 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 84.600, 84.600, 66.750 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Partially refined model created by fitting a Se-MET derived Map from same crystal form collected at synchotron radiation source. |
RMSD bond length | 0.015 |
RMSD bond angle | 1.950 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 5773 | |
<I/σ(I)> | 19.8 | |
Completeness [%] | 95.3 | 87 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | vapor diffusion under oil | 8.8 | 293 | Bicine, Ammonium Sulfate, pH 8.8, vapor diffusion under oil, temperature 293K |