1S5G
Structure of Scallop myosin S1 reveals a novel nucleotide conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.051, 50.935, 161.590 |
| Unit cell angles | 90.00, 98.36, 90.00 |
Refinement procedure
| Resolution | 43.440 - 3.100 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kk7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 21309 | |
| <I/σ(I)> | 20.6 | 4.2 |
| Completeness [%] | 85.2 | 59.1 |
| Redundancy | 4.2 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 300 | PEG 8000, magnesium chloride, ADP, Glycerol, MES, TMAO, cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
