1S5D
Cholera holotoxin with an A-subunit Y30S mutation, Crystal form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-28 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.055, 111.904, 124.656 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.710 - 1.750 |
| R-factor | 0.16278 |
| Rwork | 0.161 |
| R-free | 0.19149 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CTY30S Form 3 structure (PDB ID 1S5B) |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.227 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.920 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 81705 | |
| <I/σ(I)> | 25.3 | 3.37 |
| Completeness [%] | 95.5 | 73.2 |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | PEG 3350, sodium citrate, galactose, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
