1S4U
Crystal Structure analysis of the beta-propeller protein Ski8p
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-10 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9789, 0.9791, 0.9724 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.555, 66.791, 81.459 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.960 - 2.100 |
R-factor | 0.2529 |
Rwork | 0.251 |
R-free | 0.28332 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.060 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.154 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 21439 | |
Completeness [%] | 1.0 | 0.988 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG4000, Sodium citrate, Ethylene glycol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |