1S4K
Putative cytoplasmic protein from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2003-12-21 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97885 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 85.596, 90.258, 78.669 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.890 - 1.900 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.21900 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data reduction software | d*TREK |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 22681 | |
| <I/σ(I)> | 3.34 | |
| Completeness [%] | 86.0 | 59.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 294 | NaH2PO4, K2HPO4, acetate, sucrose, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
