1S44
The structure and refinement of apocrustacyanin C2 to 1.6A resolution and the search for differences between this protein and the homologous apoproteins A1 and C1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS |
| Synchrotron site | SRS |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2002-06-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.167, 79.879, 109.562 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.550 - 1.600 |
| R-factor | 0.20514 |
| Rwork | 0.203 |
| R-free | 0.24744 |
| Structure solution method | not applicable |
| Starting model (for MR) | 1h91 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.265 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.970 | 1.680 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.132 | 0.662 |
| Number of reflections | 47856 | |
| <I/σ(I)> | 4.7 | 1.1 |
| Completeness [%] | 98.6 | 97.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9 | 298 | 2.3M ammonium sulphate, 20% glycerol, pH 9, VAPOR DIFFUSION, temperature 298K |
