1S3S
Crystal structure of AAA ATPase p97/VCP ND1 in complex with p47 C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9394 |
| Spacegroup name | P 65 |
| Unit cell lengths | 157.719, 157.719, 243.195 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.700 - 2.900 |
| Rwork | 0.248 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1E32.pdb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.990 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.096 | 0.580 |
| Number of reflections | 75663 | |
| <I/σ(I)> | 10.5 | 3.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | PEGmme 5K, Citrate, KSCN, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
