1S3J

X-ray crystal structure of YusO protein from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-25
Detector	SBC-2
Spacegroup name	P 21 21 21
Unit cell lengths	45.029, 71.912, 96.642
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.710 - 2.250
R-factor	0.221
Rwork	0.219
R-free	0.27300
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.222
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.250	2.250
Rmerge	0.101	0.510
Number of reflections	16195
<I/σ(I)>	8
Completeness [%]	99.5	94.5
Redundancy	13.59

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	294	Ammonium Sulfate, Bis-Tris, Pentaerythritol Ethoxylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K, pH 6.50