1S3F
Purine 2'-deoxyribosyltransferase + selenoinosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 170 |
| Detector technology | CCD |
| Collection date | 2002-01-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.582, 79.582, 185.727 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.200 |
| Rwork | 0.274 |
| R-free | 0.29300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.066 | 0.256 |
| Number of reflections | 35106 | |
| <I/σ(I)> | 6.7 | 2.8 |
| Completeness [%] | 98.1 | 100 |
| Redundancy | 13 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 2.0 M ammonium sulfate, 100mM tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
