1S32
Molecular Recognition of the Nucleosomal 'Supergroove'
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.352, 109.717, 181.827 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.050 |
| Rwork | 0.219 |
| R-free | 0.24340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aoi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.099 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.0) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.076 | 0.353 |
| Number of reflections | 124523 | |
| <I/σ(I)> | 22.99 | 2.04 |
| Completeness [%] | 94.4 | 54.1 |
| Redundancy | 4.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | Manganese chloride, potassium chloride, potassium cacodylate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Manganese chloride | ||
| 2 | 1 | 1 | potassium chloride | ||
| 3 | 1 | 1 | potassium cacodylate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | Manganese chloride | ||
| 6 | 1 | 2 | potassium chloride | ||
| 7 | 1 | 2 | H2O |
