1S2O
X-Ray structure of the sucrose-phosphatase (SPP) from Synechocystis sp. PCC6803 at 1.40 A resolution
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-11 |
Detector | MARRESEARCH |
Wavelength(s) | 1.483148 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.070, 51.760, 52.140 |
Unit cell angles | 90.00, 101.70, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.400 |
Rwork | 0.182 |
R-free | 0.20000 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 1.312 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.400 |
Rmerge | 0.057 |
Number of reflections | 45838 |
<I/σ(I)> | 12.58 |
Completeness [%] | 96.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | NaFormate, NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K | |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | NaFormate, NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K | |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | NaFormate, NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |