1S21

Crystal Structure of AvrPphF ORF2, A Type III Effector from P. syringae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-08-28
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	38.689, 68.334, 88.365
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.630 - 2.000
R-factor	0.225
Rwork	0.225
R-free	0.24100
Structure solution method	MIRAS
RMSD bond length	0.005
RMSD bond angle	1.300
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.054	0.233
Number of reflections	16442
<I/σ(I)>	18.9	6.1
Completeness [%]	99.8	99.1
Redundancy	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	PEG8000, Bis-Tris, Magnesium Acetate and glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	PEG8000, Bis-Tris, Magnesium Acetate and glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K