1S1Q

TSG101(UEV) domain in complex with Ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-01-10
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97979
Spacegroup name	C 1 2 1
Unit cell lengths	143.634, 59.154, 93.998
Unit cell angles	90.00, 128.67, 90.00

Refinement procedure

Resolution	72.550 - 2.000
R-factor	0.20319
Rwork	0.201
R-free	0.23998
Structure solution method	SAD & Molecular replacement
Starting model (for MR)	1KPP & 1UBO
RMSD bond length	0.019
RMSD bond angle	1.357
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.550	2.100
High resolution limit [Å]	2.000	2.000
Rmerge		0.335
Number of reflections	40921
<I/σ(I)>		2.1
Completeness [%]	97.8	96.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	298	1.4M Ammonium sulfate, 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K