1S1K
INFLUENCE OF GROOVE INTERACTIONS ON DNA HOLLIDAY JUNCTION FORMATION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2002-01-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 32.957, 32.957, 88.556 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 17.490 - 1.900 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p4z |
RMSD bond length | 0.067 |
RMSD bond angle | 6.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.500 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.056 | 0.245 |
Number of reflections | 2528 | |
<I/σ(I)> | 4.98 | |
Completeness [%] | 96.3 | 72.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 5mM TRIS(HCl), 23mM CaAcetate, 16% MPD, .6mM DNA against 28% MPD at RT, pH 7.50, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | TRIS(HCl) | ||
2 | 1 | 1 | CaAcetate | ||
3 | 1 | 1 | 16% MPD | ||
4 | 1 | 1 | DNA | ||
5 | 1 | water | |||
6 | 1 | 28% MPD | |||
7 | 1 | TRIS(HCl) |