1S16

Crystal Structure of E. coli Topoisomerase IV ParE 43kDa subunit complexed with ADPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-12-20
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	94.900, 120.800, 136.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.2244
Rwork	0.224
R-free	0.24050
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000
High resolution limit [Å]	2.100	2.100
Number of reflections	88735
Completeness [%]	96.8	96.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	298	peg 3350 potassium chloride, sodium acetate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K