1S0U

eIF2gamma apo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	108
Detector technology	CCD
Collection date	2001-12-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97884
Spacegroup name	P 1 21 1
Unit cell lengths	52.842, 52.490, 74.134
Unit cell angles	90.00, 92.53, 90.00

Refinement procedure

Resolution	22.000 - 2.400
Rwork	0.212
R-free	0.26400
Structure solution method	SAD
RMSD bond length	0.009 *
RMSD bond angle	2.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.058 *	0.171 *
Number of reflections	15988
Completeness [%]	98.4	84.2
Redundancy	6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100mM Hepes, 10% PEG 6K, 5% v/v methyl pentanediol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	HEPES	100 (mM)	pH7.5
3	1	reservoir	PEG6000	10 (%(w/v))
4	1	reservoir	methyl pentanediol	5 (%(v/v))