1RZT
Crystal structure of DNA polymerase lambda complexed with a two nucleotide gap DNA molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-02-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 191.321, 99.034, 104.838 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 2.100 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | polymerase beta |
| RMSD bond length | 0.006 |
| RMSD bond angle | 21.500 * |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.475 * | 0.284 * |
| Total number of observations | 590501 * | |
| Number of reflections | 111222 | |
| <I/σ(I)> | 16.8 | 2.9 |
| Completeness [%] | 94.9 | 67.2 |
| Redundancy | 5.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 279 | cacodylate, sodium acetate, PEG8000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 279K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | cacodylate | ||
| 2 | 1 | 1 | Na(C2H3O2) | ||
| 3 | 1 | 2 | Na(C2H3O2) |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 18 (mg/ml) | |
| 2 | 1 | drop | dithiothreitol | 50 (mM) | pH7.5 |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | glycerol | 5 (%) | |
| 5 | 1 | drop | 0.1-0.7 (M) | ||
| 6 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.0 |
| 7 | 1 | reservoir | calcium acetate | 0.2 (M) | |
| 8 | 1 | reservoir | PEG8000 | 4 (%(w/v)) |
