1RYX
Crystal structure of hen serum transferrin in apo- form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 90.295, 90.295, 177.666 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.690 - 3.500 |
| R-factor | 0.283 |
| Rwork | 0.283 |
| R-free | 0.34100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aiv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.480 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.102 | 0.453 |
| Number of reflections | 10753 | |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 277 | 18-23% PEG 6000 in 0.02M sodium acetate buffer, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
