1RYM

Structure of the Group II Intron Splicing Factor CRS2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2001-12-06
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00
Spacegroup name	I 2 2 2
Unit cell lengths	53.100, 79.600, 112.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 * - 1.800
R-factor	0.195
Rwork	0.190
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	CRS2 residues through K178 (PTH numbering)
RMSD bond length	0.025 *
RMSD bond angle	2.800 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	EPMR
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000 *	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.069 *	0.257 *
Number of reflections	22475
<I/σ(I)>	5.7	2.5
Completeness [%]	99.9	99.9
Redundancy	6.9	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	10% PEG 3400, sodium citrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG3400	10 (%)
3	1	reservoir	sodium citrate	100 (mM)	pH5.5