1RYM
Structure of the Group II Intron Splicing Factor CRS2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 53.100, 79.600, 112.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 * - 1.800 |
| R-factor | 0.195 |
| Rwork | 0.190 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CRS2 residues through K178 (PTH numbering) |
| RMSD bond length | 0.025 * |
| RMSD bond angle | 2.800 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | EPMR |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 * | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.069 * | 0.257 * |
| Number of reflections | 22475 | |
| <I/σ(I)> | 5.7 | 2.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.9 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 10% PEG 3400, sodium citrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG3400 | 10 (%) | |
| 3 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.5 |
