1RYD
Crystal Structure of Glucose-Fructose Oxidoreductase from Zymomonas mobilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-08-22 |
Detector | SBC-2 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 84.525, 91.382, 115.473 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.680 - 2.200 |
R-factor | 0.209 |
Rwork | 0.204 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ofg |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | d*TREK |
Data scaling software | HKL-2000 |
Phasing software | EPMR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.122 | 0.560 |
Number of reflections | 45279 | |
<I/σ(I)> | 7.2 | 2.7 |
Completeness [%] | 98.6 | 94.1 |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | PEG 4000, ammonium acetate, sodium citrate, glucose, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |