1RY9

Spa15, a Type III Secretion Chaperone from Shigella flexneri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-23
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9196
Spacegroup name	P 1 21 1
Unit cell lengths	50.878, 87.682, 58.703
Unit cell angles	90.00, 90.77, 90.00

Refinement procedure

Resolution	43.850 - 1.820
R-factor	0.18272
Rwork	0.181
R-free	0.21108
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.235
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	REFMAC (5.1.27)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.890
High resolution limit [Å]	1.820	1.820
Number of reflections	45246
Completeness [%]	97.4	92.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	291	Ammonium Suplhate, ethylene glycol, Guanidium chloride, MES, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	5.3	291	Ammonium Suplhate, ethylene glycol, Guanidium chloride, MES, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	5.3	291	Ammonium Suplhate, ethylene glycol, Guanidium chloride, MES, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K