1RXT
Crystal structure of human myristoyl-CoA:protein N-myristoyltransferase.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.626, 116.425, 90.361 |
| Unit cell angles | 90.00, 90.13, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| Rwork | 0.283 |
| R-free | 0.39100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 29147 | |
| Completeness [%] | 97.0 | 92.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | ammonium sulfate, CoCl2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
