1RXQ
YfiT from Bacillus subtilis is a probable metal-dependent hydrolase with an unusual four-helix bundle topology
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2003-12-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.370, 50.420, 89.410 |
| Unit cell angles | 104.38, 90.61, 112.05 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.18798 |
| Rwork | 0.186 |
| R-free | 0.21708 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.898 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.744 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.085 | 0.298 |
| Number of reflections | 70594 | |
| <I/σ(I)> | 6.4 | 3.1 |
| Completeness [%] | 67.1 | |
| Redundancy | 6.2 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1M sodium cacodylate, 23% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
