1RXM

C-terminal region of FEN-1 bound to A. fulgidus PCNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-08-02
Detector	ADSC QUANTUM 4
Wavelength(s)	0.855
Spacegroup name	H 3 2
Unit cell lengths	101.127, 101.127, 203.148
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.211
Rwork	0.205
R-free	0.26100 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rwz
RMSD bond length	0.006
RMSD bond angle	1.290
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	11174
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	21 *	Na/K phosphate, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	drop	Tris	50 (mM)	pH8.0
3	1	drop		250 (mM)
4	1	reservoir	sodium/pootassium phosphate	1.4-1.6 (M)	pH9.0