1RXJ
Crystal structure of streptavidin mutant (M2) where the L3,4 loop was replace by that of avidin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2002-12-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.003 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.355, 85.080, 57.694 |
| Unit cell angles | 90.00, 98.17, 90.00 |
Refinement procedure
| Resolution | 56.800 - 1.140 |
| R-factor | 0.18022 |
| Rwork | 0.179 |
| R-free | 0.20029 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.247 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 56.800 |
| High resolution limit [Å] | 1.140 |
| Number of reflections | 148143 |
| <I/σ(I)> | 12.4 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 4000, 0.1M NaAc, 0.1M NaCac, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
