1RWY
CRYSTAL STRUCTURE OF RAT ALPHA-PARVALBUMIN AT 1.05 RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-17 |
| Detector | SBC-2 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.838, 54.708, 153.643 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.100 - 1.050 |
| R-factor | 0.133 |
| R-free | 0.16200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rtp |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.029 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.100 | 1.070 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.060 | 0.319 |
| Number of reflections | 132869 | |
| <I/σ(I)> | 36.2 | 4.6 |
| Completeness [%] | 99.2 | 97.5 |
| Redundancy | 7.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 298 | PEG, ammonium sulfate, sodium acetate, temperature 298K, pH 4.60 |
