1RW4

Nitrogenase Fe protein l127 deletion variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Wavelength(s)	2.5
Spacegroup name	C 2 2 21
Unit cell lengths	70.780, 133.090, 61.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.222
Rwork	0.220
R-free	0.27200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g20
RMSD bond length	0.010 *
RMSD bond angle	2.700 *
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.500
Rmerge	0.059 *	0.135 *
Total number of observations	122724 *
Number of reflections	10015
Completeness [%]	99.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	8.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	70 (mg/ml)
2	1	1	PEG4000	24 (%)
3	1	1		0.16 (M)	pH8.5
4	1	1	trishydrochloric acid
5	1	1	glycerol	20 (%)
6	1	1	sodium dithionite	1 (mM)