1RV4

E75L MUTANT OF RABBIT CYTOSOLIC SERINE HYDROXYMETHYLTRANSFERASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-06
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9363
Spacegroup name	P 41 21 2
Unit cell lengths	115.040, 115.040, 158.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.510 - 2.950
R-factor	0.202
Rwork	0.202
R-free	0.24100
Structure solution method	Rigid body refinement of another E75L rcSHMT structure
Starting model (for MR)	1rv3
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	DPS
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.500	3.110
High resolution limit [Å]	2.950	2.950
Number of reflections	23067
<I/σ(I)>	6.1	1.4
Completeness [%]	99.9	100
Redundancy	9	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	298	PEG 8000, KCl, potassium phosphate, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K