1RV3

E75L MUTANT OF RABBIT CYTOSOLIC SERINE HYDROXYMETHYLTRANSFERASE, COMPLEX WITH GLYCINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-06
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9363
Spacegroup name	P 41 21 2
Unit cell lengths	114.920, 114.920, 158.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.880 - 2.400
R-factor	0.224
Rwork	0.224
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cj0
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DPS
Data scaling software	CCP4 ((SCALA))
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.900	2.530
High resolution limit [Å]	2.400	2.400
Number of reflections	42006
<I/σ(I)>	6	1.3
Completeness [%]	99.8	99.7
Redundancy	13	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	298	PEG 8000, KCl, potassium phosphate, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K