1RTD
STRUCTURE OF A CATALYTIC COMPLEX OF HIV-1 REVERSE TRANSCRIPTASE: IMPLICATIONS FOR NUCLEOSIDE ANALOG DRUG RESISTANCE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Detector | BRANDEIS - B4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.840, 150.700, 280.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 3.200 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.100 |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.103 * | 0.372 * |
| Number of reflections | 54281 | 4802 * |
| <I/σ(I)> | 11.2 | 2.7 |
| Completeness [%] | 93.8 | 84.3 |
| Redundancy | 4.2 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 4 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein-DNA complex | 8 (mg/ml) | |
| 10 | 1 | reservoir | 10 (mM) | ||
| 11 | 1 | reservoir | EDTA | 0.1 (mM) | |
| 2 | 1 | drop | MES | 10 (mM) | |
| 3 | 1 | drop | 2 (mM) | ||
| 4 | 1 | drop | 200 (mM) | ||
| 5 | 1 | drop | EDTA | 0.02 (mM) | |
| 6 | 1 | drop | dTTP | 2.5 (mM) | |
| 7 | 1 | reservoir | PEG6000 | 14 (%(w/v)) | |
| 8 | 1 | reservoir | MES | 50 (mM) | |
| 9 | 1 | reservoir | 1 (M) |
