1RT5
HIV-1 REVERSE TRANSCRIPTASE COMPLEXED WITH UC10
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 289 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04 |
| Detector | FUJI |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 140.300, 111.000, 73.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.21 * |
| Rwork | 0.231 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RT-1051U91 COMPLEX (PDB ENTRY 1RTH) |
| RMSD bond length | 0.006 |
| RMSD bond angle | 24.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.062 | 0.447 |
| Total number of observations | 67590 * | |
| Number of reflections | 22803 | 1812 * |
| <I/σ(I)> | 11.4 | 1.05 |
| Completeness [%] | 86.1 | 73.4 |
| Redundancy | 2.96 | 1.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5 | 4 * | pH 5.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | citric acid | 24.25 (mM) | |
| 2 | 1 | 1 | sodium phosphate | 51.5 (mM) | |
| 3 | 1 | 1 | PEG3400 | 6-10 (%(w/v)) |
