1RSN

RIBONUCLEASE (RNASE SA) (E.C.3.1.4.8) COMPLEXED WITH EXO-2',3'-CYCLOPHOSPHOROTHIOATE

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Detector technology	IMAGE PLATE
Collection date	1992-11
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	64.700, 78.800, 39.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.119
RMSD bond length	0.021
RMSD bond angle	0.047
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.034
Total number of observations	48382 *
Number of reflections	13932 *
Completeness [%]	98.4 *
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.2 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3 (%)
2	1	drop	sodium phosphate	0.1 (M)
3	1	reservoir	ammonium sulfate