1RSM

THE 2-ANGSTROMS RESOLUTION STRUCTURE OF A THERMOSTABLE RIBONUCLEASE A CHEMICALLY CROSS-LINKED BETWEEN LYSINE RESIDUES 7 AND 41

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	37.050, 41.260, 75.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.000
R-factor	0.184 *
Rwork	0.184
RMSD bond length	0.024
RMSD bond angle	0.038 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	2.100 *
High resolution limit [Å]	2.000 *
Rmerge	0.081 *
Total number of observations	10701 *
Number of reflections	8301 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	8 *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ethanol	30 (%(v/v))	50mM-Tris-HCl(pH7.5) and 50 mM-imidazole(pH8.0) are also suitable